Journal: Advanced Science

Article Title: A Membrane‐Targeted Photosensitizer Prevents Drug Resistance and Induces Immune Response in Treating Candidiasis

doi: 10.1002/advs.202207736

Figure Lengend Snippet: Photodynamic eradication of C. albicans biofilms with TBTCP‐QY. A) Scheme for determining the changes in morphology and survival rates of mature C. albicans biofilms treated with or without TBTCP‐QY and light irradiation (80 mW cm −2 ) for 15 min. B) Representative 3D images of C. albicans biofilms. Thirty‐six‐hour‐old mature biofilms were cocultured with 5 µ m TBTCP‐QY or 0.12% CHX in the dark or irradiated with white light for 15 min (80 mW cm −2 ), followed by staining with a Live & Dead Viability/Cytotoxicity assay kit. A 488 nm laser and a 515–550 nm emission filter were employed for the green channel, and a 561 nm laser and a 570–620 nm emission filter were used for the red channel (scale bar: 50 µm). C) Representative FESEM images of C. albicans biofilms with different treatments. Mature biofilms were cocultured with 5 µ m TBTCP‐QY or 0.12% CHX and then kept in the dark or irradiated with white light for 15 min (80 mW cm −2 ). The red arrows indicate deformed or destroyed fungal and hyphal cell envelopes (scale bar: 20 µm and 10 µm).

Article Snippet: The 3D images were processed using NIS‐Elements AR software (Nikon) and Comstat2.

Techniques: Irradiation, Staining, Cytotoxicity Assay